Pre-mRNA splicing occurs in 2 sequential transesterification steps. The protein encoded by this gene is found to be essential for the catalytic step II in pre-mRNA splicing process. It is found in the spliceosome, and contains seven WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to the yeast splicing factor Prp18. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for PRPF18. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = PRPF18 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of PRPF18, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr10:13,299,293–13,300,384 | 287.0 kb | Distal (>10kb) Multiome | 850 | |
| chr10:13,302,089–13,302,853 | 284.6 kb | Distal (>10kb) Multiome | 807 | |
| chr10:13,347,195–13,348,918 | 238.8 kb | Distal (>10kb) Multiome | 753 | |
| chr10:13,349,410–13,350,393 | 237.0 kb | Distal (>10kb) Multiome | 432 | |
| chr10:13,351,365–13,351,877 | 235.4 kb | Distal (>10kb) Multiome | 105 | |
| chr10:13,440,173–13,440,755 | 146.4 kb | Distal (>10kb) Multiome | 414 | |
| chr10:13,528,519–13,529,578 | 57.9 kb | Distal (>10kb) Multiome | 267 | |
| chr10:13,586,728–13,587,229 | 45 bp | At TSS Multiome | 812 | |
| chr10:13,690,233–13,691,095 | 103.7 kb | Distal (>10kb) Multiome | 202 | |
| chr10:13,707,398–13,707,910 | 120.6 kb | Distal (>10kb) Multiome | 353 | |
| chr10:13,881,844–13,882,604 | 295.4 kb | Distal (>10kb) Multiome | 306 |
Genomic view of the PRPF18 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.