This gene encodes a vitamin K-dependent plasma protein that functions as a cofactor for the anticoagulant protease, activated protein C (APC) to inhibit blood coagulation. It is found in plasma in both a free, functionally active form and also in an inactive form complexed with C4b-binding protein. Mutations in this gene result in autosomal dominant hereditary thrombophilia. An inactive pseudogene of this locus is located at an adjacent region on chromosome 3. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar processing to generate mature protein. [provided by RefSeq, Oct 2015]
Transcription factors with Perturb-seq knockdown data for PROS1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = PROS1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of PROS1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr3:93,973,221–93,974,313 | 103 bp | At TSS Multiome | 755 | |
| chr3:93,979,611–93,980,934 | 6.2 kb | Proximal (<10kb) Multiome | 702 | |
| chr3:94,062,348–94,063,486 | 89.0 kb | Distal (>10kb) Multiome | 797 | |
| chr3:94,066,379–94,067,550 | 93.0 kb | Distal (>10kb) Multiome | 160 | |
| chr3:94,250,605–94,251,787 | 277.3 kb | Distal (>10kb) Multiome | 210 | |
| chr3:94,256,923–94,257,930 | 283.5 kb | Distal (>10kb) Multiome | 219 |
Genomic view of the PROS1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.