Arginine methylation is a widespread posttranslational modification mediated by arginine methyltransferases, such as PRMT8. Arginine methylation is involved in a number of cellular processes, including DNA repair, RNA transcription, signal transduction, protein compartmentalization, and possibly protein translation (Lee et al., 2005 [PubMed 16051612]).[supplied by OMIM, Mar 2008]
Transcription factors with Perturb-seq knockdown data for PRMT8. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = PRMT8 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of PRMT8, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr12:3,199,420–3,200,898 | 290.9 kb | Distal (>10kb) Multiome | 348 | |
| chr12:3,214,367–3,215,060 | 276.4 kb | Distal (>10kb) Multiome | 142 | |
| chr12:3,365,831–3,366,469 | 125.0 kb | Distal (>10kb) Multiome | 149 | |
| chr12:3,490,906–3,491,329 | at TSS | At TSS | 104 | |
| chr12:3,491,725–3,492,673 | 1.1 kb | Proximal (<10kb) Multiome | 517 | |
| chr12:3,752,872–3,753,459 | 262.0 kb | Distal (>10kb) Multiome | 419 |
Genomic view of the PRMT8 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.