The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for PRKAA1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = PRKAA1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of PRKAA1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr5:39,072,981–39,075,472 | 1723.7 kb | Distal (>10kb) Multiome HiCAR | 1130 | |
| chr5:40,678,872–40,680,670 | 118.4 kb | Distal (>10kb) Multiome | 615 | |
| chr5:40,680,710–40,682,280 | 116.5 kb | Distal (>10kb) Multiome | 343 | |
| chr5:40,754,888–40,756,381 | 42.2 kb | Distal (>10kb) Multiome | 1007 | |
| chr5:40,797,344–40,798,944 | 65 bp | At TSS Multiome | 949 | |
| chr5:40,801,606–40,802,349 | 3.4 kb | Proximal (<10kb) | 300 | |
| chr5:40,834,732–40,836,235 | 37.2 kb | Distal (>10kb) Multiome | 1118 |
Genomic view of the PRKAA1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.