The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase can interact with Rho guanine nucleotide exchange factors (PIX), and thus block the effects of p21-activated kinase 1 (PAK), a protein kinase mediating biological effects downstream of Rho GTPases. Calcium/calmodulin-dependent protein kinase II gamma (CAMK2G/CAMK-II) is found to be one of the substrates of this phosphatase. The overexpression of this phosphatase or CAMK2G has been shown to mediate caspase-dependent apoptosis. An alternatively spliced transcript variant has been identified, but its full-length nature has not been determined. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for PPM1F. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = PPM1F upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of PPM1F, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr22:21,657,073–21,658,429 | 295.2 kb | Distal (>10kb) Multiome | 802 | |
| chr22:21,665,721–21,666,577 | 286.7 kb | Distal (>10kb) Multiome | 893 | |
| chr22:21,735,345–21,736,601 | 216.9 kb | Distal (>10kb) Multiome | 774 | |
| chr22:21,864,937–21,865,391 | 87.8 kb | Distal (>10kb) Multiome | 184 | |
| chr22:21,866,936–21,868,402 | 85.0 kb | Distal (>10kb) Multiome | 929 | |
| chr22:21,925,450–21,926,079 | 27.0 kb | Distal (>10kb) Multiome | 169 | |
| chr22:21,938,040–21,938,786 | 14.6 kb | Distal (>10kb) Multiome | 871 | |
| chr22:21,947,693–21,947,938 | 4.9 kb | Proximal (<10kb) | 222 | |
| chr22:21,951,964–21,953,251 | 22 bp | At TSS Multiome | 645 |
Genomic view of the PPM1F locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.