This gene encodes a small essential subunit of RNA polymerase III, the polymerase responsible for synthesizing transfer and small ribosomal RNAs in eukaryotes. The carboxy-terminal domain of this subunit shares a high degree of sequence similarity to the carboxy-terminal domain of an RNA polymerase II elongation factor. This similarity in sequence is supported by functional studies showing that this subunit is required for proper pausing and termination during transcription. Pseudogenes of this gene are found on chromosomes 13 and 17.[provided by RefSeq, Jul 2010]
Transcription factors with Perturb-seq knockdown data for POLR3K. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = POLR3K upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of POLR3K, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr16:52,954–54,208 | 117 bp | At TSS Multiome | 950 | |
| chr16:71,378–73,062 | 17.9 kb | Distal (>10kb) Multiome | 639 | |
| chr16:77,240–78,636 | 24.5 kb | Distal (>10kb) Multiome | 685 | |
| chr16:138,551–139,186 | 85.1 kb | Distal (>10kb) Multiome | 674 | |
| chr16:164,983–166,147 | 111.5 kb | Distal (>10kb) Multiome | 234 | |
| chr16:180,313–181,713 | 127.9 kb | Distal (>10kb) Multiome | 994 | |
| chr16:228,869–229,598 | 175.7 kb | Distal (>10kb) Multiome | 1035 | |
| chr16:234,152–235,669 | 181.2 kb | Distal (>10kb) Multiome | 908 | |
| chr16:351,804–354,436 | 299.3 kb | Distal (>10kb) Multiome | 654 |
Genomic view of the POLR3K locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.