This gene encodes the 66-kDa subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. The encoded protein plays a role in regulating the activity of DNA polymerase delta through interactions with other subunits and the processivity cofactor proliferating cell nuclear antigen (PCNA). Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Mar 2012]
Transcription factors with Perturb-seq knockdown data for POLD3. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = POLD3 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of POLD3, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr11:74,311,252–74,311,993 | 182.2 kb | Distal (>10kb) Multiome | 289 | |
| chr11:74,397,796–74,399,222 | 95.2 kb | Distal (>10kb) Multiome | 811 | |
| chr11:74,467,393–74,467,865 | 26.4 kb | Distal (>10kb) Multiome | 174 | |
| chr11:74,492,667–74,494,411 | 434 bp | At TSS Multiome | 897 | |
| chr11:74,592,212–74,593,000 | 98.7 kb | Distal (>10kb) Multiome | 716 | |
| chr11:74,731,155–74,731,658 | 237.6 kb | Distal (>10kb) Multiome | 131 | |
| chr11:74,748,226–74,749,731 | 255.0 kb | Distal (>10kb) Multiome | 854 |
Genomic view of the POLD3 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.