The protein encoded by this gene belongs to the protein kinase C superfamily. This kinase is activated by Rho family of small G proteins and may mediate the Rho-dependent signaling pathway. This kinase can be activated by phospholipids and by limited proteolysis. The 3-phosphoinositide dependent protein kinase-1 (PDPK1/PDK1) is reported to phosphorylate this kinase, which may mediate insulin signals to the actin cytoskeleton. The proteolytic activation of this kinase by caspase-3 or related proteases during apoptosis suggests its role in signal transduction related to apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for PKN1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = PKN1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of PKN1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr19:14,136,079–14,137,770 | 296.6 kb | Distal (>10kb) Multiome | 978 | |
| chr19:14,206,623–14,207,699 | 226.3 kb | Distal (>10kb) Multiome | 444 | |
| chr19:14,247,378–14,248,008 | 185.9 kb | Distal (>10kb) Multiome | 109 | |
| chr19:14,248,166–14,249,635 | 184.7 kb | Distal (>10kb) Multiome | 230 | |
| chr19:14,264,813–14,266,130 | 168.0 kb | Distal (>10kb) Multiome | 223 | |
| chr19:14,294,831–14,295,420 | 138.3 kb | Distal (>10kb) Multiome | 60 | |
| chr19:14,363,590–14,364,020 | 69.8 kb | Distal (>10kb) Multiome | 402 | |
| chr19:14,405,442–14,406,893 | 27.3 kb | Distal (>10kb) Multiome | 662 | |
| chr19:14,418,847–14,419,795 | 14.1 kb | Distal (>10kb) Multiome | 817 | |
| chr19:14,431,960–14,432,195 | 1.3 kb | Proximal (<10kb) | 300 | |
| chr19:14,432,848–14,433,780 | 142 bp | At TSS Multiome | 524 | |
| chr19:14,450,232–14,451,000 | 17.1 kb | Distal (>10kb) Multiome | 271 | |
| chr19:14,472,177–14,472,986 | 39.1 kb | Distal (>10kb) Multiome | 566 | |
| chr19:14,495,705–14,496,690 | 62.8 kb | Distal (>10kb) Multiome | 813 | |
| chr19:14,517,523–14,519,160 | 85.1 kb | Distal (>10kb) Multiome | 952 | |
| chr19:14,528,857–14,530,279 | 96.0 kb | Distal (>10kb) Multiome | 842 | |
| chr19:14,536,700–14,537,683 | 103.7 kb | Distal (>10kb) Multiome | 329 | |
| chr19:14,556,545–14,557,088 | 123.3 kb | Distal (>10kb) Multiome | 509 | |
| chr19:14,564,060–14,565,155 | 131.0 kb | Distal (>10kb) Multiome | 226 | |
| chr19:14,571,772–14,572,330 | 138.6 kb | Distal (>10kb) Multiome | 896 | |
| chr19:14,689,407–14,690,497 | 256.5 kb | Distal (>10kb) Multiome | 624 |
Genomic view of the PKN1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.