This gene encodes a member of the protein kinase inhibitor family. Studies of a similar protein in mice suggest that this protein acts as a potent competitive cAMP-dependent protein kinase inhibitor, and is a predominant form of inhibitor in various tissues. The encoded protein may be involved in osteogenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
Transcription factors with Perturb-seq knockdown data for PKIG. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = PKIG upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of PKIG, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr20:44,310,737–44,311,623 | 220.7 kb | Distal (>10kb) Multiome | 908 | |
| chr20:44,475,692–44,476,318 | 56.0 kb | Distal (>10kb) Multiome | 962 | |
| chr20:44,521,574–44,522,457 | 9.9 kb | Proximal (<10kb) Multiome | 937 | |
| chr20:44,522,935–44,523,165 | 8.7 kb | Proximal (<10kb) | 24 | |
| chr20:44,523,577–44,525,342 | 7.0 kb | Proximal (<10kb) Multiome | 231 | |
| chr20:44,531,682–44,532,180 | 29 bp | At TSS Multiome | 880 | |
| chr20:44,609,775–44,610,281 | 78.2 kb | Distal (>10kb) Multiome | 727 | |
| chr20:44,642,246–44,643,140 | 110.8 kb | Distal (>10kb) Multiome | 469 | |
| chr20:44,651,099–44,652,334 | 119.9 kb | Distal (>10kb) Multiome | 641 | |
| chr20:44,745,652–44,746,316 | 214.0 kb | Distal (>10kb) Multiome | 321 | |
| chr20:44,810,403–44,810,885 | 278.6 kb | Distal (>10kb) Multiome | 181 |
Genomic view of the PKIG locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.