Spermatogenesis is a complex process regulated by extracellular and intracellular factors as well as cellular interactions among interstitial cells of the testis, Sertoli cells, and germ cells. This gene is expressed in the testis in Sertoli cells but not germ cells. The protein encoded by this gene contains plant homeodomain (PHD) finger domains, also known as leukemia associated protein (LAP) domains, believed to be involved in transcriptional regulation. The protein, which localizes to the nucleus of transfected cells, has been implicated in the transcriptional regulation of spermatogenesis. Alternate splicing results in multiple transcript variants of this gene. [provided by RefSeq, May 2013]
Transcription factors with Perturb-seq knockdown data for PHF7. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = PHF7 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of PHF7, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr3:52,128,194–52,128,700 | 282.3 kb | Distal (>10kb) Multiome | 376 | |
| chr3:52,153,766–52,155,196 | 256.0 kb | Distal (>10kb) Multiome | 896 | |
| chr3:52,197,554–52,198,856 | 212.6 kb | Distal (>10kb) Multiome | 731 | |
| chr3:52,238,767–52,239,716 | 171.4 kb | Distal (>10kb) Multiome | 763 | |
| chr3:52,245,446–52,246,450 | 164.7 kb | Distal (>10kb) Multiome | 553 | |
| chr3:52,277,664–52,279,184 | 132.0 kb | Distal (>10kb) Multiome | 844 | |
| chr3:52,287,499–52,288,336 | 122.7 kb | Distal (>10kb) Multiome | 778 | |
| chr3:52,409,430–52,411,187 | 355 bp | At TSS Multiome | 813 | |
| chr3:52,412,177–52,412,350 | 1.5 kb | Proximal (<10kb) | 116 | |
| chr3:52,444,941–52,445,411 | 34.5 kb | Distal (>10kb) Multiome HiCAR | 426 | |
| chr3:52,455,213–52,455,878 | 44.9 kb | Distal (>10kb) Multiome | 630 | |
| chr3:52,518,607–52,519,389 | 108.3 kb | Distal (>10kb) Multiome | 176 | |
| chr3:52,533,486–52,535,316 | 123.3 kb | Distal (>10kb) Multiome | 648 | |
| chr3:52,536,232–52,537,450 | 125.9 kb | Distal (>10kb) Multiome | 901 | |
| chr3:52,685,427–52,686,463 | 275.1 kb | Distal (>10kb) Multiome | 933 | |
| chr3:52,705,495–52,706,415 | 295.3 kb | Distal (>10kb) Multiome | 862 |
Genomic view of the PHF7 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.