In Drosophila melanogaster, the 'Polycomb' group (PcG) of genes are part of a cellular memory system that is responsible for the stable inheritance of gene activity. PcG proteins form a large multimeric, chromatin-associated protein complex. The protein encoded by this gene has homology to the Drosophila PcG protein 'polyhomeotic' (Ph) and is known to heterodimerize with EDR1 and colocalize with BMI1 in interphase nuclei of human cells. The specific function in human cells has not yet been determined. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for PHC2. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = PHC2 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of PHC2, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:33,080,764–33,081,783 | 268.7 kb | Distal (>10kb) Multiome | 556 | |
| chr1:33,127,180–33,127,648 | 222.4 kb | Distal (>10kb) Multiome | 877 | |
| chr1:33,152,752–33,153,392 | 196.7 kb | Distal (>10kb) Multiome | 151 | |
| chr1:33,180,551–33,182,306 | 168.9 kb | Distal (>10kb) Multiome | 692 | |
| chr1:33,255,428–33,256,795 | 94.1 kb | Distal (>10kb) Multiome | 509 | |
| chr1:33,338,430–33,338,960 | 11.1 kb | Distal (>10kb) Multiome | 254 | |
| chr1:33,342,108–33,342,803 | 7.3 kb | Proximal (<10kb) Multiome | 722 | |
| chr1:33,349,195–33,350,690 | 150 bp | At TSS Multiome | 874 | |
| chr1:33,363,845–33,364,278 | 14.3 kb | Distal (>10kb) Multiome | 214 | |
| chr1:33,430,392–33,431,511 | 81.3 kb | Distal (>10kb) Multiome | 555 | |
| chr1:33,472,141–33,472,925 | 122.8 kb | Distal (>10kb) Multiome | 656 |
Genomic view of the PHC2 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.