This gene encodes a member of the prefoldin alpha subunit family. The encoded protein is one of six subunits of prefoldin, a molecular chaperone complex that binds and stabilizes newly synthesized polypeptides, thereby allowing them to fold correctly. The complex, consisting of two alpha and four beta subunits, forms a double beta barrel assembly with six protruding coiled-coils. The encoded protein may also repress the transcriptional activity of the proto-oncogene c-Myc. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for PFDN5. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = PFDN5 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of PFDN5, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr12:53,005,438–53,007,356 | 289.2 kb | Distal (>10kb) Multiome | 995 | |
| chr12:53,046,385–53,049,295 | 248.1 kb | Distal (>10kb) Multiome | 793 | |
| chr12:53,049,465–53,050,530 | 245.6 kb | Distal (>10kb) Multiome | 565 | |
| chr12:53,053,508–53,054,860 | 241.1 kb | Distal (>10kb) Multiome | 646 | |
| chr12:53,078,790–53,079,991 | 216.2 kb | Distal (>10kb) Multiome | 835 | |
| chr12:53,097,544–53,098,160 | 197.8 kb | Distal (>10kb) Multiome | 515 | |
| chr12:53,174,900–53,175,484 | 120.3 kb | Distal (>10kb) Multiome | 474 | |
| chr12:53,179,987–53,181,456 | 114.8 kb | Distal (>10kb) Multiome | 930 | |
| chr12:53,219,787–53,222,151 | 74.4 kb | Distal (>10kb) Multiome HiCAR | 828 | |
| chr12:53,231,703–53,233,098 | 63.2 kb | Distal (>10kb) Multiome | 464 | |
| chr12:53,251,461–53,252,566 | 43.5 kb | Distal (>10kb) Multiome HiCAR | 825 | |
| chr12:53,268,046–53,268,600 | 27.3 kb | Distal (>10kb) Multiome | 709 | |
| chr12:53,295,142–53,295,823 | 89 bp | At TSS Multiome | 706 | |
| chr12:53,299,515–53,300,187 | 4.2 kb | Proximal (<10kb) Multiome | 849 | |
| chr12:53,321,051–53,322,029 | 25.9 kb | Distal (>10kb) Multiome | 935 | |
| chr12:53,324,682–53,326,037 | 29.4 kb | Distal (>10kb) Multiome | 840 | |
| chr12:53,344,427–53,345,660 | 49.4 kb | Distal (>10kb) Multiome | 645 | |
| chr12:53,370,910–53,371,893 | 75.9 kb | Distal (>10kb) Multiome | 861 | |
| chr12:53,378,839–53,381,297 | 85.0 kb | Distal (>10kb) Multiome | 895 | |
| chr12:53,381,689–53,382,262 | 86.5 kb | Distal (>10kb) Multiome | 264 | |
| chr12:53,421,253–53,422,044 | 126.1 kb | Distal (>10kb) Multiome | 431 | |
| chr12:53,440,979–53,441,838 | 146.1 kb | Distal (>10kb) Multiome | 779 | |
| chr12:53,451,563–53,453,067 | 156.5 kb | Distal (>10kb) Multiome | 999 | |
| chr12:53,492,147–53,494,149 | 197.6 kb | Distal (>10kb) Multiome HiCAR | 702 | |
| chr12:53,498,714–53,500,111 | 203.9 kb | Distal (>10kb) Multiome | 454 | |
| chr12:53,500,242–53,501,839 | 205.5 kb | Distal (>10kb) Multiome | 1010 |
Genomic view of the PFDN5 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.