This gene encodes a family member of serine/threonine p21-activating kinases, known as PAK proteins. These proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling, and they serve as targets for the small GTP binding proteins Cdc42 and Rac. This specific family member regulates cell motility and morphology. Mutations in this gene have been associated with macrocephaly, seizures, and speech delay. Overexpression of this gene is also reported in many cancer types, and particularly in breast cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2020]
Transcription factors with Perturb-seq knockdown data for PAK1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = PAK1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of PAK1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr11:77,126,985–77,127,575 | 346.8 kb | Distal (>10kb) Multiome | 458 | |
| chr11:77,147,671–77,148,349 | 326.1 kb | Distal (>10kb) Multiome | 172 | |
| chr11:77,171,758–77,172,392 | 302.0 kb | Distal (>10kb) Multiome | 275 | |
| chr11:77,191,860–77,192,374 | 281.9 kb | Distal (>10kb) Multiome HiCAR | 447 | |
| chr11:77,411,314–77,412,382 | 62.1 kb | Distal (>10kb) Multiome | 405 | |
| chr11:77,436,791–77,437,318 | 36.9 kb | Distal (>10kb) Multiome | 44 | |
| chr11:77,473,004–77,474,837 | 58 bp | At TSS Multiome | 769 | |
| chr11:77,555,382–77,556,090 | 81.8 kb | Distal (>10kb) Multiome | 138 | |
| chr11:77,589,259–77,590,628 | 115.7 kb | Distal (>10kb) Multiome | 830 | |
| chr11:77,637,444–77,638,090 | 163.7 kb | Distal (>10kb) Multiome | 929 |
Genomic view of the PAK1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.