Transcription factors with Perturb-seq knockdown data for OSER1-DT. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = OSER1-DT upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of OSER1-DT, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr20:43,914,353–43,915,171 | 296.1 kb | Distal (>10kb) Multiome | 285 | |
| chr20:43,977,883–43,978,419 | 232.7 kb | Distal (>10kb) Multiome | 84 | |
| chr20:44,031,571–44,032,501 | 178.9 kb | Distal (>10kb) Multiome | 106 | |
| chr20:44,210,101–44,211,557 | 6 bp | At TSS Multiome | 1054 | |
| chr20:44,217,440–44,217,612 | 6.5 kb | Proximal (<10kb) | 318 | |
| chr20:44,310,737–44,311,623 | 100.3 kb | Distal (>10kb) Multiome HiCAR | 908 | |
| chr20:44,475,692–44,476,318 | 265.0 kb | Distal (>10kb) Multiome HiCAR | 962 |
Genomic view of the OSER1-DT locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.