The protein encoded by this gene is located in the nuclear envelope. It has protein similarity to nuclear transport factor 2. This protein functions as a nuclear export factor in both RAN (Ras-related nuclear protein)- and CRM1 (required for chromosome region maintenance)-dependent pathways. It is found to stimulate the export of U1 snRNA in RAN- and CRM1-dependent pathways and the export of tRNA and mRNA in a CRM1-independent pathway. The encoded protein heterodimerizes with Tap protein and may regulate the ability of Tap protein to mediate nuclear mRNA export. The use of alternate polyadenylation sites has been found for this gene. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for NXT1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = NXT1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of NXT1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr20:21,702,756–21,703,845 | 1647.6 kb | Distal (>10kb) Multiome HiCAR | 201 | |
| chr20:21,705,595–21,706,927 | 1644.8 kb | Distal (>10kb) Multiome HiCAR | 161 | |
| chr20:22,571,104–22,572,289 | 779.2 kb | Distal (>10kb) Multiome HiCAR | 192 | |
| chr20:22,574,659–22,577,435 | 775.8 kb | Distal (>10kb) Multiome HiCAR | 577 | |
| chr20:22,577,666–22,580,215 | 771.5 kb | Distal (>10kb) Multiome HiCAR | 716 | |
| chr20:22,663,225–22,664,137 | 687.1 kb | Distal (>10kb) Multiome HiCAR | 211 | |
| chr20:23,043,312–23,044,169 | 307.0 kb | Distal (>10kb) Multiome HiCAR | 150 | |
| chr20:23,048,356–23,051,688 | 302.1 kb | Distal (>10kb) Multiome HiCAR | 568 | |
| chr20:23,102,752–23,103,227 | 247.9 kb | Distal (>10kb) Multiome | 47 | |
| chr20:23,178,656–23,179,482 | 171.8 kb | Distal (>10kb) Multiome | 198 | |
| chr20:23,211,541–23,212,970 | 138.4 kb | Distal (>10kb) Multiome | 321 | |
| chr20:23,340,615–23,340,806 | 10.0 kb | Proximal (<10kb) | 173 | |
| chr20:23,349,714–23,351,638 | 213 bp | At TSS Multiome | 1037 | |
| chr20:23,355,682–23,356,065 | 4.9 kb | Proximal (<10kb) | 17 | |
| chr20:23,356,179–23,356,563 | 5.4 kb | Proximal (<10kb) | 95 | |
| chr20:23,357,172–23,358,689 | 7.4 kb | Proximal (<10kb) Multiome | 873 | |
| chr20:23,361,395–23,363,116 | 11.3 kb | Distal (>10kb) Multiome | 1092 | |
| chr20:23,420,902–23,421,923 | 70.7 kb | Distal (>10kb) Multiome | 823 | |
| chr20:23,637,267–23,638,378 | 287.2 kb | Distal (>10kb) Multiome | 884 |
Genomic view of the NXT1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.