N-ethylmaleimide-sensitive factor (NSF) and valosin-containing protein (p97) are two ATPases known to be involved in transport vesicle/target membrane fusion and fusions between membrane compartments. A trimer of the protein encoded by this gene binds a hexamer of cytosolic p97 and is required for p97-mediated regrowth of Golgi cisternae from mitotic Golgi fragments. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 8. [provided by RefSeq, May 2011]
Transcription factors with Perturb-seq knockdown data for NSFL1C. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = NSFL1C upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of NSFL1C, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr20:1,184,818–1,186,197 | 281.1 kb | Distal (>10kb) Multiome | 557 | |
| chr20:1,225,365–1,226,806 | 241.0 kb | Distal (>10kb) Multiome | 616 | |
| chr20:1,266,029–1,266,958 | 200.4 kb | Distal (>10kb) Multiome | 499 | |
| chr20:1,300,911–1,301,500 | 165.6 kb | Distal (>10kb) Multiome | 171 | |
| chr20:1,313,163–1,313,790 | 153.3 kb | Distal (>10kb) Multiome HiCAR | 428 | |
| chr20:1,325,008–1,326,107 | 141.4 kb | Distal (>10kb) Multiome | 901 | |
| chr20:1,392,628–1,393,457 | 73.7 kb | Distal (>10kb) Multiome | 799 | |
| chr20:1,427,438–1,428,145 | 39.1 kb | Distal (>10kb) Multiome | 285 | |
| chr20:1,466,316–1,467,471 | 64 bp | At TSS Multiome | 1054 | |
| chr20:1,470,126–1,470,371 | 3.3 kb | Proximal (<10kb) | 523 | |
| chr20:1,475,180–1,475,519 | 8.3 kb | Proximal (<10kb) | 70 |
Genomic view of the NSFL1C locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.