Transcription factors with Perturb-seq knockdown data for NKILA. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = NKILA upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of NKILA, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr20:57,596,872–57,597,457 | 113.0 kb | Distal (>10kb) Multiome | 473 | |
| chr20:57,705,582–57,705,980 | 4.2 kb | Proximal (<10kb) | 34 | |
| chr20:57,709,617–57,711,153 | 18 bp | At TSS Multiome | 630 | |
| chr20:57,711,888–57,712,329 | 1.7 kb | Proximal (<10kb) | 42 | |
| chr20:57,718,453–57,719,417 | 8.6 kb | Proximal (<10kb) Multiome | 444 |
Genomic view of the NKILA locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.