This nuclear gene encodes a conserved protein that comprises the B13 subunit of complex I of the mitochondrial respiratory chain. The encoded protein localizes to the inner mitochondrial membrane, where it is thought to aid in the transfer of electrons from NADH to ubiquinone. Alternative splicing results in multiple transcript variants. There are numerous pseudogenes of this gene on chromosomes 1, 3, 6, 8, 9, 11, 12, and 16. [provided by RefSeq, Apr 2014]
Transcription factors with Perturb-seq knockdown data for NDUFA5. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = NDUFA5 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of NDUFA5, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr7:123,284,880–123,285,858 | 272.4 kb | Distal (>10kb) Multiome | 233 | |
| chr7:123,533,937–123,535,843 | 23.0 kb | Distal (>10kb) Multiome | 560 | |
| chr7:123,557,224–123,558,428 | 69 bp | At TSS Multiome | 732 | |
| chr7:123,594,565–123,595,316 | 37.1 kb | Distal (>10kb) Multiome | 183 | |
| chr7:123,637,247–123,638,386 | 80.2 kb | Distal (>10kb) Multiome | 95 | |
| chr7:123,657,889–123,659,811 | 100.6 kb | Distal (>10kb) Multiome | 165 | |
| chr7:123,692,360–123,692,863 | 134.8 kb | Distal (>10kb) Multiome | 179 | |
| chr7:123,747,604–123,749,779 | 191.3 kb | Distal (>10kb) Multiome | 1065 |
Genomic view of the NDUFA5 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.