This gene encodes a member of the nuclear distribution E (NudE) family of proteins. The encoded protein is localized at the centrosome and interacts with other centrosome components as part of a multiprotein complex that regulates dynein function. This protein plays an essential role in microtubule organization, mitosis and neuronal migration. Mutations in this gene cause lissencephaly 4, a disorder characterized by lissencephaly, severe brain atrophy, microcephaly, and severe cognitive disability. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2012]
Transcription factors with Perturb-seq knockdown data for NDE1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = NDE1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of NDE1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr16:15,546,648–15,547,130 | 96.4 kb | Distal (>10kb) Multiome | 20 | |
| chr16:15,590,892–15,591,343 | 52.2 kb | Distal (>10kb) Multiome | 798 | |
| chr16:15,642,538–15,643,550 | 120 bp | At TSS Multiome | 945 | |
| chr16:15,649,782–15,650,732 | 6.9 kb | Proximal (<10kb) Multiome | 776 | |
| chr16:15,811,511–15,812,234 | 168.6 kb | Distal (>10kb) Multiome | 195 | |
| chr16:15,888,121–15,889,301 | 245.5 kb | Distal (>10kb) Multiome | 785 | |
| chr16:15,949,200–15,949,652 | 306.2 kb | Distal (>10kb) Multiome | 385 |
Genomic view of the NDE1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.