Contributes to small GTPase binding activity. Involved in Rac protein signal transduction; positive regulation of Arp2/3 complex-mediated actin nucleation; and positive regulation of lamellipodium assembly. Located in extracellular exosome and focal adhesion. Part of SCAR complex. [provided by Alliance of Genome Resources, Jul 2025]
Transcription factors with Perturb-seq knockdown data for NCKAP1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = NCKAP1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of NCKAP1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr2:182,865,796–182,867,716 | 171.2 kb | Distal (>10kb) Multiome | 424 | |
| chr2:182,929,762–182,930,671 | 108.1 kb | Distal (>10kb) Multiome | 149 | |
| chr2:183,037,288–183,039,108 | 139 bp | At TSS Multiome | 915 | |
| chr2:183,070,887–183,071,694 | 32.9 kb | Distal (>10kb) Multiome | 230 | |
| chr2:183,078,264–183,079,391 | 40.4 kb | Distal (>10kb) Multiome | 744 | |
| chr2:183,123,970–183,125,139 | 86.1 kb | Distal (>10kb) Multiome | 982 | |
| chr2:183,291,337–183,292,555 | 253.9 kb | Distal (>10kb) Multiome | 173 |
Genomic view of the NCKAP1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.