NAPEPLD is a phospholipase D type enzyme that catalyzes the release of N-acylethanolamine (NAE) from N-acyl-phosphatidylethanolamine (NAPE) in the second step of the biosynthesis of N-acylethanolamine (Okamoto et al., 2004 [PubMed 14634025]).[supplied by OMIM, Oct 2008]
Transcription factors with Perturb-seq knockdown data for NAPEPLD. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = NAPEPLD upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of NAPEPLD, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr7:103,074,047–103,075,916 | 74.6 kb | Distal (>10kb) Multiome | 860 | |
| chr7:103,148,730–103,149,910 | 234 bp | At TSS Multiome | 1002 | |
| chr7:103,279,769–103,280,793 | 130.9 kb | Distal (>10kb) Multiome | 291 | |
| chr7:103,297,040–103,297,853 | 147.9 kb | Distal (>10kb) Multiome | 880 | |
| chr7:103,344,148–103,345,138 | 195.2 kb | Distal (>10kb) Multiome | 994 | |
| chr7:103,347,517–103,348,162 | 198.2 kb | Distal (>10kb) Multiome | 795 | |
| chr7:103,445,161–103,446,559 | 296.5 kb | Distal (>10kb) Multiome | 424 |
Genomic view of the NAPEPLD locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.