NADK catalyzes the transfer of a phosphate group from ATP to NAD to generate NADP, which in its reduced form acts as an electron donor for biosynthetic reactions (Lerner et al., 2001 [PubMed 11594753]).[supplied by OMIM, Mar 2008]
Transcription factors with Perturb-seq knockdown data for NADK. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = NADK upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of NADK, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:1,511,442–1,512,404 | 266.4 kb | Distal (>10kb) Multiome | 897 | |
| chr1:1,540,164–1,541,317 | 237.8 kb | Distal (>10kb) Multiome | 195 | |
| chr1:1,573,904–1,575,432 | 203.6 kb | Distal (>10kb) Multiome | 949 | |
| chr1:1,599,945–1,600,642 | 178.4 kb | Distal (>10kb) Multiome | 307 | |
| chr1:1,615,103–1,616,799 | 162.9 kb | Distal (>10kb) Multiome | 951 | |
| chr1:1,628,379–1,632,529 | 148.3 kb | Distal (>10kb) Multiome | 552 | |
| chr1:1,658,722–1,659,418 | 119.3 kb | Distal (>10kb) Multiome | 602 | |
| chr1:1,724,054–1,724,738 | 54.0 kb | Distal (>10kb) Multiome | 631 | |
| chr1:1,777,637–1,780,173 | 329 bp | At TSS Multiome | 970 | |
| chr1:1,782,008–1,783,229 | 3.9 kb | Proximal (<10kb) Multiome | 590 | |
| chr1:1,883,184–1,883,831 | 105.2 kb | Distal (>10kb) Multiome HiCAR | 303 | |
| chr1:1,890,319–1,891,952 | 112.9 kb | Distal (>10kb) Multiome HiCAR | 748 | |
| chr1:1,908,308–1,910,312 | 130.8 kb | Distal (>10kb) Multiome HiCAR | 801 | |
| chr1:1,918,258–1,920,500 | 141.0 kb | Distal (>10kb) Multiome HiCAR | 820 | |
| chr1:1,959,901–1,960,517 | 181.8 kb | Distal (>10kb) Multiome | 594 | |
| chr1:2,019,137–2,019,982 | 241.1 kb | Distal (>10kb) Multiome | 288 | |
| chr1:2,044,569–2,045,304 | 266.6 kb | Distal (>10kb) Multiome | 403 | |
| chr1:2,049,277–2,050,992 | 271.9 kb | Distal (>10kb) Multiome | 732 |
Genomic view of the NADK locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.