Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S5P family. Pseudogenes corresponding to this gene are found on chromosomes 4q, 5q, and 18q. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for MRPS5. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = MRPS5 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of MRPS5, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr2:95,076,602–95,077,695 | 45.0 kb | Distal (>10kb) Multiome | 585 | |
| chr2:95,115,409–95,115,645 | 6.2 kb | Proximal (<10kb) | 289 | |
| chr2:95,121,334–95,122,514 | 47 bp | At TSS Multiome | 934 | |
| chr2:95,158,615–95,160,452 | 37.8 kb | Distal (>10kb) Multiome | 539 | |
| chr2:95,165,117–95,166,301 | 43.7 kb | Distal (>10kb) Multiome | 931 | |
| chr2:95,206,705–95,208,058 | 85.5 kb | Distal (>10kb) Multiome | 713 | |
| chr2:95,345,216–95,347,558 | 223.7 kb | Distal (>10kb) Multiome | 572 | |
| chr2:95,388,847–95,389,649 | 267.3 kb | Distal (>10kb) Multiome | 269 | |
| chr2:95,401,933–95,403,542 | 280.7 kb | Distal (>10kb) Multiome | 885 |
Genomic view of the MRPS5 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.