Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S14P family. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2010]
Transcription factors with Perturb-seq knockdown data for MRPS14. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = MRPS14 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of MRPS14, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:174,799,347–174,800,510 | 223.7 kb | Distal (>10kb) Multiome | 456 | |
| chr1:174,882,897–174,883,845 | 140.0 kb | Distal (>10kb) Multiome | 184 | |
| chr1:174,976,347–174,977,217 | 46.6 kb | Distal (>10kb) Multiome | 181 | |
| chr1:174,998,565–175,000,705 | 23.6 kb | Distal (>10kb) Multiome | 1242 | |
| chr1:175,022,930–175,024,023 | 91 bp | At TSS Multiome | 878 | |
| chr1:175,029,423–175,030,223 | 6.5 kb | Proximal (<10kb) Multiome | 562 | |
| chr1:175,073,610–175,074,084 | 50.4 kb | Distal (>10kb) Multiome | 76 | |
| chr1:175,129,648–175,130,843 | 106.8 kb | Distal (>10kb) Multiome | 246 | |
| chr1:175,146,568–175,147,254 | 123.4 kb | Distal (>10kb) Multiome HiCAR | 324 | |
| chr1:175,151,904–175,152,560 | 128.8 kb | Distal (>10kb) Multiome | 185 | |
| chr1:175,154,371–175,155,390 | 131.6 kb | Distal (>10kb) Multiome | 333 | |
| chr1:175,191,731–175,193,773 | 169.5 kb | Distal (>10kb) Multiome | 565 | |
| chr1:175,308,000–175,309,187 | 285.3 kb | Distal (>10kb) Multiome | 116 |
Genomic view of the MRPS14 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.