Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Multiple transcript variants encoding two different isoforms were identified through sequence analysis. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for MRPL55. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = MRPL55 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of MRPL55, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:227,946,348–227,948,809 | 162.6 kb | Distal (>10kb) Multiome | 645 | |
| chr1:228,004,351–228,004,883 | 104.7 kb | Distal (>10kb) Multiome | 154 | |
| chr1:228,058,680–228,061,126 | 49.5 kb | Distal (>10kb) Multiome | 377 | |
| chr1:228,081,634–228,083,952 | 26.7 kb | Distal (>10kb) Multiome | 942 | |
| chr1:228,085,140–228,085,832 | 23.8 kb | Distal (>10kb) Multiome | 276 | |
| chr1:228,101,882–228,103,743 | 7.1 kb | Proximal (<10kb) Multiome | 929 | |
| chr1:228,108,924–228,110,105 | 106 bp | At TSS Multiome | 958 | |
| chr1:228,130,684–228,131,457 | 21.8 kb | Distal (>10kb) Multiome | 488 | |
| chr1:228,139,579–228,140,870 | 30.8 kb | Distal (>10kb) Multiome | 931 | |
| chr1:228,164,898–228,166,840 | 56.5 kb | Distal (>10kb) Multiome | 1011 | |
| chr1:228,174,477–228,174,893 | 65.3 kb | Distal (>10kb) Multiome | 254 | |
| chr1:228,212,247–228,214,390 | 104.4 kb | Distal (>10kb) Multiome | 604 | |
| chr1:228,275,524–228,276,595 | 166.8 kb | Distal (>10kb) Multiome | 428 | |
| chr1:228,378,133–228,379,597 | 269.3 kb | Distal (>10kb) Multiome | 515 | |
| chr1:228,406,106–228,407,568 | 297.7 kb | Distal (>10kb) Multiome | 753 |
Genomic view of the MRPL55 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.