Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a putative 39S subunit protein and belongs to the L47P ribosomal protein family. Pseudogenes corresponding to this gene are found on chromosomes 2p, 2q, 5p, and 10q. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for MRPL50. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = MRPL50 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of MRPL50, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr9:100,637,622–100,638,453 | 760.7 kb | Distal (>10kb) Multiome HiCAR | 112 | |
| chr9:101,125,125–101,125,610 | 273.3 kb | Distal (>10kb) Multiome | 73 | |
| chr9:101,134,832–101,136,539 | 262.6 kb | Distal (>10kb) Multiome | 150 | |
| chr9:101,397,915–101,399,348 | 110 bp | At TSS Multiome | 861 | |
| chr9:101,449,087–101,449,796 | 50.7 kb | Distal (>10kb) Multiome | 494 | |
| chr9:101,458,960–101,459,540 | 60.6 kb | Distal (>10kb) Multiome | 253 | |
| chr9:101,460,651–101,461,628 | 62.5 kb | Distal (>10kb) Multiome | 103 | |
| chr9:101,485,920–101,487,719 | 88.4 kb | Distal (>10kb) Multiome | 692 | |
| chr9:101,533,168–101,534,447 | 135.1 kb | Distal (>10kb) Multiome | 723 |
Genomic view of the MRPL50 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.