Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Alternative splicing results in multiple transcript variants. Pseudogenes corresponding to this gene are found on chromosomes 2p and 17q. [provided by RefSeq, May 2013]
Transcription factors with Perturb-seq knockdown data for MRPL45. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = MRPL45 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of MRPL45, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr17:36,482,565–36,483,505 | 1814.0 kb | Distal (>10kb) Multiome HiCAR | 734 | |
| chr17:36,486,066–36,487,171 | 1810.5 kb | Distal (>10kb) Multiome HiCAR | 719 | |
| chr17:38,256,154–38,256,660 | 40.9 kb | Distal (>10kb) Multiome | 119 | |
| chr17:38,296,821–38,297,250 | at TSS | At TSS | 343 | |
| chr17:38,351,522–38,352,472 | 54.7 kb | Distal (>10kb) Multiome | 809 | |
| chr17:38,414,746–38,415,878 | 117.7 kb | Distal (>10kb) Multiome | 792 | |
| chr17:38,428,075–38,428,868 | 131.3 kb | Distal (>10kb) Multiome | 298 | |
| chr17:38,453,611–38,454,219 | 156.7 kb | Distal (>10kb) Multiome | 168 | |
| chr17:38,509,961–38,510,926 | 213.4 kb | Distal (>10kb) Multiome | 409 | |
| chr17:38,558,106–38,558,652 | 261.2 kb | Distal (>10kb) Multiome | 631 | |
| chr17:38,559,205–38,559,900 | 262.5 kb | Distal (>10kb) Multiome | 598 | |
| chr17:38,572,553–38,573,232 | 275.8 kb | Distal (>10kb) Multiome | 231 |
Genomic view of the MRPL45 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.