Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. This gene and the gene for a semaphorin class 4 protein (SEMA4G) overlap at map location 10q24.31 and are transcribed in opposite directions. Sequence analysis identified multiple transcript variants encoding at least four different protein isoforms. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for MRPL43. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = MRPL43 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of MRPL43, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr10:100,715,555–100,715,963 | 271.6 kb | Distal (>10kb) Multiome | 117 | |
| chr10:100,729,074–100,730,178 | 258.0 kb | Distal (>10kb) Multiome | 304 | |
| chr10:100,739,174–100,740,373 | 247.7 kb | Distal (>10kb) Multiome | 205 | |
| chr10:100,744,447–100,746,357 | 242.1 kb | Distal (>10kb) Multiome | 379 | |
| chr10:100,760,518–100,761,080 | 226.6 kb | Distal (>10kb) Multiome | 105 | |
| chr10:100,787,902–100,788,519 | 199.3 kb | Distal (>10kb) Multiome | 52 | |
| chr10:100,826,838–100,828,148 | 159.5 kb | Distal (>10kb) Multiome | 327 | |
| chr10:100,912,455–100,913,519 | 74.6 kb | Distal (>10kb) Multiome | 906 | |
| chr10:100,968,950–100,970,567 | 18.1 kb | Distal (>10kb) Multiome | 681 | |
| chr10:100,987,003–100,987,794 | 69 bp | At TSS Multiome | 786 | |
| chr10:100,994,403–100,994,731 | 6.9 kb | Proximal (<10kb) | 101 | |
| chr10:100,996,424–100,998,696 | 9.5 kb | Proximal (<10kb) Multiome | 945 | |
| chr10:100,998,810–101,000,165 | 12.1 kb | Distal (>10kb) Multiome | 845 | |
| chr10:101,018,377–101,019,461 | 31.6 kb | Distal (>10kb) Multiome | 654 | |
| chr10:101,030,588–101,032,521 | 43.7 kb | Distal (>10kb) Multiome | 613 | |
| chr10:101,042,527–101,043,387 | 55.4 kb | Distal (>10kb) Multiome | 655 | |
| chr10:101,048,051–101,048,547 | 60.8 kb | Distal (>10kb) Multiome | 87 | |
| chr10:101,049,966–101,050,753 | 62.9 kb | Distal (>10kb) Multiome | 412 | |
| chr10:101,060,073–101,063,211 | 75.3 kb | Distal (>10kb) Multiome | 1124 | |
| chr10:101,066,181–101,066,983 | 79.0 kb | Distal (>10kb) Multiome | 734 | |
| chr10:101,067,105–101,068,123 | 80.4 kb | Distal (>10kb) Multiome | 643 | |
| chr10:101,095,368–101,096,269 | 108.2 kb | Distal (>10kb) Multiome | 326 | |
| chr10:101,131,094–101,132,387 | 143.9 kb | Distal (>10kb) Multiome | 289 | |
| chr10:101,139,407–101,140,520 | 152.3 kb | Distal (>10kb) Multiome | 158 | |
| chr10:101,161,678–101,162,270 | 174.4 kb | Distal (>10kb) Multiome | 336 | |
| chr10:101,214,601–101,215,378 | 227.5 kb | Distal (>10kb) Multiome | 302 | |
| chr10:101,217,392–101,218,117 | 230.2 kb | Distal (>10kb) Multiome | 203 | |
| chr10:101,236,527–101,237,332 | 249.5 kb | Distal (>10kb) Multiome | 123 | |
| chr10:101,282,747–101,283,297 | 295.5 kb | Distal (>10kb) Multiome | 74 | |
| chr10:101,285,534–101,286,046 | 298.4 kb | Distal (>10kb) Multiome | 150 |
Genomic view of the MRPL43 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.