The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to microtubular structures in the cytoplasm. Alternate splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Feb 2009]
Transcription factors with Perturb-seq knockdown data for MID2. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = MID2 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of MID2, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chrX:107,628,178–107,629,161 | 197.4 kb | Distal (>10kb) Multiome | 409 | |
| chrX:107,654,846–107,655,657 | 170.6 kb | Distal (>10kb) Multiome | 393 | |
| chrX:107,736,604–107,737,564 | 88.8 kb | Distal (>10kb) Multiome | 303 | |
| chrX:107,825,723–107,827,075 | at TSS | At TSS | 369 | |
| chrX:108,091,177–108,092,245 | 265.7 kb | Distal (>10kb) Multiome | 574 |
Genomic view of the MID2 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.