Transcription factors with Perturb-seq knockdown data for MHENCR. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = MHENCR upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of MHENCR, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr20:63,409,631–63,410,124 | 217.3 kb | Distal (>10kb) Multiome | 60 | |
| chr20:63,471,533–63,472,788 | 154.6 kb | Distal (>10kb) Multiome | 219 | |
| chr20:63,488,166–63,488,632 | 138.8 kb | Distal (>10kb) Multiome | 127 | |
| chr20:63,498,850–63,499,391 | 127.9 kb | Distal (>10kb) Multiome | 232 | |
| chr20:63,519,657–63,521,677 | 106.6 kb | Distal (>10kb) Multiome | 712 | |
| chr20:63,537,759–63,538,455 | 89.1 kb | Distal (>10kb) Multiome | 823 | |
| chr20:63,552,551–63,554,733 | 73.6 kb | Distal (>10kb) Multiome | 544 | |
| chr20:63,573,969–63,574,596 | 52.9 kb | Distal (>10kb) Multiome | 551 | |
| chr20:63,626,480–63,627,647 | 189 bp | At TSS Multiome | 982 | |
| chr20:63,641,266–63,642,913 | 14.8 kb | Distal (>10kb) Multiome | 691 | |
| chr20:63,652,233–63,654,166 | 26.4 kb | Distal (>10kb) Multiome | 1011 | |
| chr20:63,657,813–63,658,939 | 31.2 kb | Distal (>10kb) Multiome | 1055 | |
| chr20:63,707,489–63,708,488 | 80.7 kb | Distal (>10kb) Multiome | 1008 | |
| chr20:63,737,573–63,741,470 | 112.4 kb | Distal (>10kb) Multiome | 1098 | |
| chr20:63,775,216–63,775,997 | 148.3 kb | Distal (>10kb) Multiome | 257 | |
| chr20:63,830,341–63,832,721 | 204.1 kb | Distal (>10kb) Multiome | 628 | |
| chr20:63,864,234–63,865,991 | 237.9 kb | Distal (>10kb) Multiome | 1158 | |
| chr20:63,894,341–63,895,764 | 267.8 kb | Distal (>10kb) Multiome | 791 |
Genomic view of the MHENCR locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.