This gene encodes a member of the alpha/beta hydrolase superfamily. It is imprinted, exhibiting preferential expression from the paternal allele in fetal tissues, and isoform-specific imprinting in lymphocytes. The loss of imprinting of this gene has been linked to certain types of cancer and may be due to promotor switching. The encoded protein may play a role in development. Alternatively spliced transcript variants encoding multiple isoforms have been identified for this gene. Pseudogenes of this gene are located on the short arm of chromosomes 3 and 4, and the long arm of chromosomes 6 and 15. [provided by RefSeq, Dec 2011]
Transcription factors with Perturb-seq knockdown data for MEST. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = MEST upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of MEST, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr7:130,069,977–130,071,012 | 415.9 kb | Distal (>10kb) Multiome HiCAR | 873 | |
| chr7:130,204,919–130,205,826 | 280.9 kb | Distal (>10kb) Multiome | 874 | |
| chr7:130,272,868–130,273,649 | 212.9 kb | Distal (>10kb) Multiome | 220 | |
| chr7:130,440,505–130,441,456 | 45.2 kb | Distal (>10kb) Multiome | 666 | |
| chr7:130,485,879–130,487,031 | 25 bp | At TSS Multiome | 696 | |
| chr7:130,490,902–130,491,116 | 4.6 kb | Proximal (<10kb) | 108 | |
| chr7:130,491,258–130,491,648 | 4.9 kb | Proximal (<10kb) | 91 | |
| chr7:130,491,946–130,492,475 | 5.6 kb | Proximal (<10kb) | 133 | |
| chr7:130,575,139–130,575,863 | 89.0 kb | Distal (>10kb) Multiome | 72 | |
| chr7:130,667,922–130,669,330 | 182.3 kb | Distal (>10kb) Multiome | 738 | |
| chr7:130,733,079–130,734,492 | 247.5 kb | Distal (>10kb) Multiome HiCAR | 383 |
Genomic view of the MEST locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.