Predicted to enable actin binding activity. Predicted to be involved in RNA polymerase II preinitiation complex assembly; positive regulation of transcription elongation by RNA polymerase II; and positive regulation of transcription initiation by RNA polymerase II. Predicted to act upstream of or within negative regulation of smooth muscle cell differentiation and somatic stem cell population maintenance. Located in nucleoplasm. Part of core mediator complex. [provided by Alliance of Genome Resources, Jul 2025]
Transcription factors with Perturb-seq knockdown data for MED28. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = MED28 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of MED28, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr4:17,511,108–17,512,753 | 102.5 kb | Distal (>10kb) Multiome | 724 | |
| chr4:17,576,452–17,578,383 | 37.4 kb | Distal (>10kb) Multiome | 874 | |
| chr4:17,613,634–17,615,353 | 24 bp | At TSS Multiome | 740 | |
| chr4:17,636,469–17,637,277 | 22.3 kb | Distal (>10kb) Multiome | 322 | |
| chr4:17,780,838–17,782,239 | 167.0 kb | Distal (>10kb) Multiome | 374 | |
| chr4:17,810,219–17,811,664 | 196.2 kb | Distal (>10kb) Multiome | 935 |
Genomic view of the MED28 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.