The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 5. [provided by RefSeq, Dec 2011]
Transcription factors with Perturb-seq knockdown data for MED27. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = MED27 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of MED27, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr9:131,807,487–131,807,943 | 272.1 kb | Distal (>10kb) Multiome | 104 | |
| chr9:132,006,745–132,007,281 | 72.8 kb | Distal (>10kb) Multiome | 166 | |
| chr9:132,079,578–132,080,009 | 27 bp | At TSS Multiome | 537 | |
| chr9:132,239,978–132,242,316 | 161.4 kb | Distal (>10kb) Multiome | 555 | |
| chr9:132,259,091–132,259,539 | 179.5 kb | Distal (>10kb) Multiome | 443 | |
| chr9:132,354,395–132,355,656 | 275.2 kb | Distal (>10kb) Multiome | 895 |
Genomic view of the MED27 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.