MED20

mediator complex subunit 20 | DKFZp586D2223, PRO0213, SRB2, TRFP

This gene encodes a component of the mediator complex (also known as TRAP, SMCC, DRIP, or ARC), a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. A mutation in this gene has been associated with a novel infantile-onset neurodegenerative movement disorder. Alternatively spliced transcript variants have been identified. [provided by RefSeq, Mar 2015]

Developmental clusters: GC5

Biological processes 19 terms

DNA-directed RNA polymerase activity (GO:0003899)DNA-templated transcription (GO:0006351)RNA polymerase II preinitiation complex assembly (GO:0051123)mediator complex (GO:0016592)mediator complex (GO:0016592)mediator complex (GO:0016592)nucleoplasm (GO:0005654)nucleus (GO:0005634)nucleus (GO:0005634)positive regulation of transcription elongation by RNA polymerase II (GO:0032968)positive regulation of transcription initiation by RNA polymerase II (GO:0060261)protein binding (GO:0005515)protein-containing complex (GO:0032991)regulation of transcription by RNA polymerase II (GO:0006357)regulation of transcription by RNA polymerase II (GO:0006357)regulation of transcription by RNA polymerase II (GO:0006357)transcription by RNA polymerase II (GO:0006366)transcription coactivator activity (GO:0003713)transcription coregulator activity (GO:0003712)

Expression (TPM)

TFs regulating MED20 0 TFs

Transcription factors with Perturb-seq knockdown data for MED20. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = MED20 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.

TF	Mean coef	Binding	Outlier	TF→Gene link

Rows:

Elements linked to MED20

Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of MED20, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.

Element	Dist. to TSS	Link type	TFs
chr6:41,638,107–41,639,488	282.6 kb	Distal (>10kb) Multiome	240
chr6:41,683,281–41,684,037	237.4 kb	Distal (>10kb) Multiome	492
chr6:41,733,652–41,736,107	186.9 kb	Distal (>10kb) Multiome	908
chr6:41,779,509–41,781,116	141.0 kb	Distal (>10kb) Multiome HiCAR	857
chr6:41,786,100–41,787,779	134.6 kb	Distal (>10kb) Multiome HiCAR	1106
chr6:41,894,347–41,896,221	25.7 kb	Distal (>10kb) Multiome	822
chr6:41,920,834–41,921,901	146 bp	At TSS Multiome	955
chr6:41,939,116–41,939,782	18.3 kb	Distal (>10kb) Multiome	401
chr6:41,940,689–41,942,238	20.7 kb	Distal (>10kb) Multiome	798
chr6:41,965,433–41,966,075	44.6 kb	Distal (>10kb) Multiome	152
chr6:42,030,637–42,031,169	109.9 kb	Distal (>10kb) Multiome HiCAR	297
chr6:42,048,292–42,049,051	127.6 kb	Distal (>10kb) Multiome HiCAR	627
chr6:42,050,080–42,050,921	129.5 kb	Distal (>10kb) Multiome	912
chr6:42,104,179–42,104,979	183.3 kb	Distal (>10kb) Multiome	486
chr6:42,136,520–42,137,140	215.7 kb	Distal (>10kb) Multiome	555
chr6:42,142,113–42,142,966	221.4 kb	Distal (>10kb) Multiome	489
chr6:42,166,489–42,167,048	245.6 kb	Distal (>10kb) Multiome	451
chr6:42,177,902–42,178,547	257.1 kb	Distal (>10kb) Multiome	161

Genome Browser

Genomic view of the MED20 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.