This gene encodes a nuclear protein that contains a p53 binding domain at the N-terminus and a RING finger domain at the C-terminus, and shows structural similarity to p53-binding protein MDM2. Both proteins bind the p53 tumor suppressor protein and inhibit its activity, and have been shown to be overexpressed in a variety of human cancers. However, unlike MDM2 which degrades p53, this protein inhibits p53 by binding its transcriptional activation domain. This protein also interacts with MDM2 protein via the RING finger domain, and inhibits the latter's degradation. So this protein can reverse MDM2-targeted degradation of p53, while maintaining suppression of p53 transactivation and apoptotic functions. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Feb 2011]
Transcription factors with Perturb-seq knockdown data for MDM4. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = MDM4 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of MDM4, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:203,795,422–203,796,461 | 720.7 kb | Distal (>10kb) Multiome HiCAR | 784 | |
| chr1:204,287,567–204,288,449 | 228.3 kb | Distal (>10kb) Multiome | 373 | |
| chr1:204,289,294–204,289,779 | 226.9 kb | Distal (>10kb) Multiome | 126 | |
| chr1:204,350,954–204,351,460 | 165.2 kb | Distal (>10kb) Multiome | 138 | |
| chr1:204,363,217–204,363,961 | 152.7 kb | Distal (>10kb) Multiome | 524 | |
| chr1:204,377,771–204,378,537 | 138.2 kb | Distal (>10kb) Multiome | 593 | |
| chr1:204,411,136–204,412,704 | 104.5 kb | Distal (>10kb) Multiome | 939 | |
| chr1:204,417,015–204,417,887 | 98.9 kb | Distal (>10kb) Multiome | 590 | |
| chr1:204,446,280–204,446,835 | 69.9 kb | Distal (>10kb) Multiome | 311 | |
| chr1:204,493,614–204,495,352 | 21.7 kb | Distal (>10kb) Multiome | 876 | |
| chr1:204,506,146–204,507,240 | 9.8 kb | Proximal (<10kb) Multiome | 889 | |
| chr1:204,507,371–204,508,288 | 8.4 kb | Proximal (<10kb) Multiome | 508 | |
| chr1:204,515,995–204,516,830 | 24 bp | At TSS Multiome | 1084 | |
| chr1:204,565,653–204,566,244 | 49.6 kb | Distal (>10kb) Multiome | 403 | |
| chr1:204,684,550–204,685,936 | 169.1 kb | Distal (>10kb) Multiome | 313 | |
| chr1:204,709,678–204,711,587 | 194.1 kb | Distal (>10kb) Multiome | 521 | |
| chr1:204,714,466–204,715,865 | 198.7 kb | Distal (>10kb) Multiome | 356 | |
| chr1:204,806,979–204,807,672 | 290.8 kb | Distal (>10kb) Multiome | 641 |
Genomic view of the MDM4 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.