The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the mini-chromosome maintenance proteins is a key component of the pre-replication complex and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein contains the central domain that is conserved among the mini-chromosome maintenance proteins. The encoded protein may interact with other mini-chromosome maintenance proteins and play a role in DNA replication. This gene may be associated with length of reproductive lifespan and menopause. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2013]
Transcription factors with Perturb-seq knockdown data for MCM8. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = MCM8 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of MCM8, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr20:5,750,092–5,750,988 | 200.2 kb | Distal (>10kb) Multiome | 748 | |
| chr20:5,911,112–5,912,073 | 39.0 kb | Distal (>10kb) Multiome | 998 | |
| chr20:5,950,060–5,951,226 | 67 bp | At TSS Multiome | 919 | |
| chr20:6,005,701–6,006,981 | 55.4 kb | Distal (>10kb) Multiome | 999 | |
| chr20:6,051,939–6,053,048 | 101.9 kb | Distal (>10kb) Multiome | 308 | |
| chr20:6,058,492–6,059,323 | 108.2 kb | Distal (>10kb) Multiome | 284 | |
| chr20:6,122,311–6,123,822 | 172.5 kb | Distal (>10kb) Multiome | 952 |
Genomic view of the MCM8 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.