The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of the complex by CDC2 kinase reduces the helicase activity, suggesting a role in the regulation of DNA replication. Single nucleotide polymorphisms in the intron regions of this gene are associated with differential transcriptional activation of the promoter of the neighboring lactase gene and, thereby, influence lactose intolerance in early adulthood. [provided by RefSeq, May 2012]
Transcription factors with Perturb-seq knockdown data for MCM6. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = MCM6 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of MCM6, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr2:135,741,176–135,742,744 | 134.7 kb | Distal (>10kb) Multiome | 978 | |
| chr2:135,874,180–135,874,845 | 1.6 kb | Proximal (<10kb) | 45 | |
| chr2:135,875,648–135,876,919 | 4 bp | At TSS Multiome | 849 | |
| chr2:135,984,898–135,986,233 | 109.2 kb | Distal (>10kb) Multiome | 1057 | |
| chr2:135,997,201–135,997,833 | 121.1 kb | Distal (>10kb) Multiome | 160 | |
| chr2:136,025,017–136,026,218 | 149.2 kb | Distal (>10kb) Multiome | 322 | |
| chr2:136,116,129–136,119,923 | 243.0 kb | Distal (>10kb) Multiome | 623 | |
| chr2:136,125,768–136,126,914 | 249.9 kb | Distal (>10kb) Multiome | 196 |
Genomic view of the MCM6 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.