The protein encoded by the classic MBP gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, MBP-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long MBP gene (otherwise called "Golli-MBP") that contains 3 additional exons located upstream of the classic MBP exons. Alternative splicing from the Golli and the MBP transcription start sites gives rise to 2 sets of MBP-related transcripts and gene products. The Golli mRNAs contain 3 exons unique to Golli-MBP, spliced in-frame to 1 or more MBP exons. They encode hybrid proteins that have N-terminal Golli aa sequence linked to MBP aa sequence. The second family of transcripts contain only MBP exons and produce the well characterized myelin basic proteins. This complex gene structure is conserved among species suggesting that the MBP transcription unit is an integral part of the Golli transcription unit and that this arrangement is important for the function and/or regulation of these genes. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for MBP. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = MBP upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of MBP, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr18:76,983,122–76,983,873 | 149.3 kb | Distal (>10kb) Multiome | 312 | |
| chr18:77,088,896–77,089,706 | 43.4 kb | Distal (>10kb) Multiome | 303 | |
| chr18:77,099,611–77,100,346 | 32.8 kb | Distal (>10kb) Multiome | 134 | |
| chr18:77,124,640–77,124,825 | 7.9 kb | Proximal (<10kb) | 157 | |
| chr18:77,131,101–77,131,562 | 1.2 kb | Proximal (<10kb) | 218 | |
| chr18:77,131,704–77,133,800 | 20 bp | At TSS Multiome | 561 | |
| chr18:77,249,343–77,251,937 | 117.1 kb | Distal (>10kb) Multiome | 221 |
Genomic view of the MBP locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.