This gene encodes a member of the serine/threonine protein kinase family. This kinase contains a leucine-zipper domain and is predominately expressed in neuronal cells. The phosphorylation state of this kinase in synaptic terminals was shown to be regulated by membrane depolarization via calcineurin. This kinase forms heterodimers with leucine zipper containing transcription factors, such as cAMP responsive element binding protein (CREB) and MYC, and thus may play a regulatory role in PKA or retinoic acid induced neuronal differentiation. Alternatively spliced transcript variants encoding different proteins have been described.[provided by RefSeq, Jul 2010]
Transcription factors with Perturb-seq knockdown data for MAP3K12. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = MAP3K12 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of MAP3K12, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr12:53,219,787–53,222,151 | 278.5 kb | Distal (>10kb) Multiome | 828 | |
| chr12:53,231,703–53,233,098 | 267.3 kb | Distal (>10kb) Multiome | 464 | |
| chr12:53,251,461–53,252,566 | 247.6 kb | Distal (>10kb) Multiome | 825 | |
| chr12:53,268,046–53,268,600 | 231.4 kb | Distal (>10kb) Multiome | 709 | |
| chr12:53,295,142–53,295,823 | 204.2 kb | Distal (>10kb) Multiome HiCAR | 706 | |
| chr12:53,299,515–53,300,187 | 199.9 kb | Distal (>10kb) Multiome HiCAR | 849 | |
| chr12:53,321,051–53,322,029 | 178.1 kb | Distal (>10kb) Multiome HiCAR | 935 | |
| chr12:53,324,682–53,326,037 | 174.7 kb | Distal (>10kb) Multiome HiCAR | 840 | |
| chr12:53,344,427–53,345,660 | 154.6 kb | Distal (>10kb) Multiome HiCAR | 645 | |
| chr12:53,370,910–53,371,893 | 128.2 kb | Distal (>10kb) Multiome | 861 | |
| chr12:53,378,839–53,381,297 | 119.1 kb | Distal (>10kb) Multiome | 895 | |
| chr12:53,381,689–53,382,262 | 117.6 kb | Distal (>10kb) Multiome | 264 | |
| chr12:53,421,253–53,422,044 | 78.0 kb | Distal (>10kb) Multiome | 431 | |
| chr12:53,440,979–53,441,838 | 58.0 kb | Distal (>10kb) Multiome | 779 | |
| chr12:53,451,563–53,453,067 | 47.6 kb | Distal (>10kb) Multiome | 999 | |
| chr12:53,492,147–53,494,149 | 6.5 kb | Proximal (<10kb) Multiome | 702 | |
| chr12:53,496,158–53,496,570 | 2.9 kb | Proximal (<10kb) | 188 | |
| chr12:53,498,714–53,500,111 | 155 bp | At TSS Multiome | 454 | |
| chr12:53,500,242–53,501,839 | 1.4 kb | Proximal (<10kb) Multiome | 1010 | |
| chr12:53,508,612–53,509,050 | 9.2 kb | Proximal (<10kb) | 366 | |
| chr12:53,625,664–53,626,787 | 126.6 kb | Distal (>10kb) Multiome | 880 | |
| chr12:53,675,553–53,677,839 | 176.5 kb | Distal (>10kb) Multiome | 1104 | |
| chr12:53,696,162–53,696,648 | 196.9 kb | Distal (>10kb) Multiome | 228 | |
| chr12:53,727,002–53,728,055 | 228.0 kb | Distal (>10kb) Multiome | 768 | |
| chr12:53,738,393–53,738,964 | 239.1 kb | Distal (>10kb) Multiome | 131 | |
| chr12:53,746,454–53,746,954 | 247.2 kb | Distal (>10kb) Multiome | 503 | |
| chr12:53,756,873–53,757,721 | 257.7 kb | Distal (>10kb) Multiome | 367 |
Genomic view of the MAP3K12 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.