The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically interacts with and activates MAPK7/ERK5. This kinase itself can be phosphorylated and activated by MAP3K3/MEKK3, as well as by atypical protein kinase C isoforms (aPKCs). The signal cascade mediated by this kinase is involved in growth factor stimulated cell proliferation and muscle cell differentiation. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been described. [provided by RefSeq, May 2011]
Transcription factors with Perturb-seq knockdown data for MAP2K5. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = MAP2K5 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of MAP2K5, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr15:67,254,178–67,255,165 | 288.0 kb | Distal (>10kb) Multiome | 964 | |
| chr15:67,267,516–67,268,376 | 274.8 kb | Distal (>10kb) Multiome | 61 | |
| chr15:67,474,546–67,475,121 | 68.0 kb | Distal (>10kb) Multiome | 32 | |
| chr15:67,520,813–67,522,002 | 21.4 kb | Distal (>10kb) Multiome | 1071 | |
| chr15:67,542,323–67,543,328 | 54 bp | At TSS Multiome | 734 | |
| chr15:67,548,971–67,549,180 | 6.3 kb | Proximal (<10kb) | 173 | |
| chr15:67,782,016–67,782,994 | 239.6 kb | Distal (>10kb) Multiome HiCAR | 131 | |
| chr15:67,825,208–67,826,124 | 282.8 kb | Distal (>10kb) Multiome | 173 | |
| chr15:67,839,939–67,840,842 | 297.6 kb | Distal (>10kb) Multiome | 902 |
Genomic view of the MAP2K5 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.