The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Multiple alternatively spliced transcript variants that encode distinct isoforms have been reported for this gene. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for MAP2K3. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = MAP2K3 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of MAP2K3, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr17:20,992,594–20,993,594 | 291.5 kb | Distal (>10kb) Multiome | 237 | |
| chr17:21,041,818–21,044,206 | 241.4 kb | Distal (>10kb) Multiome | 1019 | |
| chr17:21,098,970–21,099,982 | 185.3 kb | Distal (>10kb) Multiome | 514 | |
| chr17:21,106,347–21,107,024 | 178.0 kb | Distal (>10kb) Multiome | 32 | |
| chr17:21,108,710–21,109,234 | 175.8 kb | Distal (>10kb) Multiome | 13 | |
| chr17:21,126,572–21,127,535 | 157.7 kb | Distal (>10kb) Multiome | 707 | |
| chr17:21,213,670–21,215,538 | 70.3 kb | Distal (>10kb) Multiome | 849 | |
| chr17:21,252,548–21,253,969 | 31.3 kb | Distal (>10kb) Multiome | 558 | |
| chr17:21,275,381–21,276,434 | 8.7 kb | Proximal (<10kb) Multiome | 671 | |
| chr17:21,280,014–21,280,480 | 4.5 kb | Proximal (<10kb) Multiome | 435 | |
| chr17:21,283,767–21,285,795 | 97 bp | At TSS Multiome | 672 | |
| chr17:21,322,850–21,323,584 | 38.5 kb | Distal (>10kb) Multiome | 172 | |
| chr17:21,376,013–21,377,629 | 91.9 kb | Distal (>10kb) Multiome | 253 | |
| chr17:21,456,975–21,457,864 | 172.5 kb | Distal (>10kb) Multiome | 423 | |
| chr17:21,511,340–21,512,139 | 227.0 kb | Distal (>10kb) Multiome | 291 |
Genomic view of the MAP2K3 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.