Transcription factors with Perturb-seq knockdown data for MAN2A1-DT. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = MAN2A1-DT upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of MAN2A1-DT, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr5:109,685,876–109,686,194 | 2.1 kb | Proximal (<10kb) | 14 | |
| chr5:109,688,464–109,691,494 | 135 bp | At TSS | 1024 | |
| chr5:109,692,866–109,693,093 | 4.5 kb | Proximal (<10kb) | 44 | |
| chr5:109,694,328–109,694,645 | 6.0 kb | Proximal (<10kb) | 51 | |
| chr5:109,697,362–109,697,593 | 9.0 kb | Proximal (<10kb) | 41 |
Genomic view of the MAN2A1-DT locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.