This gene encodes a large protein containing numerous spectrin and leucine-rich repeat (LRR) domains. The encoded protein is a member of a family of proteins that form bridges between different cytoskeletal elements. This protein facilitates actin-microtubule interactions at the cell periphery and couples the microtubule network to cellular junctions. Alternative splicing results in multiple transcript variants, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, May 2013]
Transcription factors with Perturb-seq knockdown data for MACF1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = MACF1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of MACF1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:38,817,720–38,818,696 | 287.1 kb | Distal (>10kb) Multiome | 375 | |
| chr1:38,859,087–38,860,584 | 245.6 kb | Distal (>10kb) Multiome | 848 | |
| chr1:38,872,771–38,873,884 | 232.0 kb | Distal (>10kb) Multiome | 778 | |
| chr1:38,990,541–38,992,051 | 114.2 kb | Distal (>10kb) Multiome | 902 | |
| chr1:39,025,981–39,026,877 | 79.0 kb | Distal (>10kb) Multiome | 808 | |
| chr1:39,080,764–39,081,936 | 24.2 kb | Distal (>10kb) Multiome | 352 | |
| chr1:39,098,618–39,098,942 | 6.5 kb | Proximal (<10kb) | 272 | |
| chr1:39,102,611–39,102,809 | 2.6 kb | Proximal (<10kb) | 259 | |
| chr1:39,104,780–39,106,278 | 370 bp | At TSS Multiome | 621 | |
| chr1:39,132,955–39,133,390 | 27.8 kb | Distal (>10kb) Multiome | 384 | |
| chr1:39,276,096–39,276,654 | 171.0 kb | Distal (>10kb) Multiome | 93 | |
| chr1:39,394,478–39,395,744 | 290.3 kb | Distal (>10kb) Multiome | 295 | |
| chr1:39,408,101–39,409,740 | 303.4 kb | Distal (>10kb) Multiome | 760 |
Genomic view of the MACF1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.