This gene encodes the B subunit of lactate dehydrogenase enzyme, which catalyzes the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+ in a post-glycolysis process. Alternatively spliced transcript variants have been found for this gene. Recent studies have shown that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes. Mutations in this gene are associated with lactate dehydrogenase B deficiency. Pseudogenes have been identified on chromosomes X, 5 and 13. [provided by RefSeq, Feb 2016]
Transcription factors with Perturb-seq knockdown data for LDHB. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = LDHB upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of LDHB, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr12:21,369,286–21,369,990 | 288.2 kb | Distal (>10kb) Multiome | 118 | |
| chr12:21,437,461–21,438,474 | 220.1 kb | Distal (>10kb) Multiome | 804 | |
| chr12:21,500,928–21,502,428 | 156.1 kb | Distal (>10kb) Multiome | 804 | |
| chr12:21,527,079–21,528,319 | 130.0 kb | Distal (>10kb) Multiome | 422 | |
| chr12:21,656,935–21,658,449 | 34 bp | At TSS Multiome | 715 | |
| chr12:21,659,493–21,659,640 | 1.7 kb | Proximal (<10kb) | 11 | |
| chr12:21,774,351–21,775,580 | 117.0 kb | Distal (>10kb) Multiome | 443 | |
| chr12:22,436,588–22,437,692 | 779.4 kb | Distal (>10kb) Multiome HiCAR | 80 |
Genomic view of the LDHB locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.