This gene is a member of the Src family of protein tyrosine kinases (PTKs). The encoded protein is a key signaling molecule in the selection and maturation of developing T-cells. It contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to the plasma membrane and pericentrosomal vesicles, and binds to cell surface receptors, including CD4 and CD8, and other signaling molecules. Multiple alternatively spliced variants encoding different isoforms have been described. [provided by RefSeq, Aug 2016]
Transcription factors with Perturb-seq knockdown data for LCK. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = LCK upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of LCK, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:32,013,168–32,015,085 | 260.4 kb | Distal (>10kb) Multiome | 988 | |
| chr1:32,072,319–32,073,738 | 200.9 kb | Distal (>10kb) Multiome | 909 | |
| chr1:32,107,881–32,108,706 | 166.0 kb | Distal (>10kb) Multiome | 779 | |
| chr1:32,179,263–32,180,190 | 94.3 kb | Distal (>10kb) Multiome | 1019 | |
| chr1:32,200,233–32,201,756 | 73.4 kb | Distal (>10kb) Multiome | 573 | |
| chr1:32,204,985–32,206,258 | 68.6 kb | Distal (>10kb) Multiome | 679 | |
| chr1:32,221,877–32,222,889 | 51.8 kb | Distal (>10kb) Multiome | 755 | |
| chr1:32,239,728–32,240,979 | 33.8 kb | Distal (>10kb) Multiome | 456 | |
| chr1:32,248,081–32,248,895 | 25.6 kb | Distal (>10kb) Multiome | 622 | |
| chr1:32,273,787–32,274,188 | at TSS | At TSS | 175 | |
| chr1:32,291,710–32,292,726 | 18.0 kb | Distal (>10kb) Multiome | 900 | |
| chr1:32,334,713–32,336,836 | 62.1 kb | Distal (>10kb) Multiome | 804 | |
| chr1:32,351,055–32,352,355 | 77.4 kb | Distal (>10kb) Multiome | 944 | |
| chr1:32,361,365–32,362,459 | 87.9 kb | Distal (>10kb) Multiome | 305 | |
| chr1:32,393,928–32,395,356 | 120.5 kb | Distal (>10kb) Multiome | 930 | |
| chr1:32,437,702–32,438,502 | 164.2 kb | Distal (>10kb) Multiome | 85 | |
| chr1:32,464,697–32,465,552 | 190.9 kb | Distal (>10kb) Multiome | 408 | |
| chr1:32,500,363–32,501,049 | 226.6 kb | Distal (>10kb) Multiome | 540 | |
| chr1:32,520,498–32,520,989 | 246.6 kb | Distal (>10kb) Multiome | 384 | |
| chr1:32,539,285–32,540,081 | 265.6 kb | Distal (>10kb) Multiome | 434 |
Genomic view of the LCK locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.