This gene encodes one of the vertebrate laminin alpha chains. Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. The protein encoded by this gene is the alpha-5 subunit of of laminin-10 (laminin-511), laminin-11 (laminin-521) and laminin-15 (laminin-523). [provided by RefSeq, Jun 2013]
Transcription factors with Perturb-seq knockdown data for LAMA5. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = LAMA5 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of LAMA5, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr20:62,121,331–62,123,419 | 245.1 kb | Distal (>10kb) Multiome | 961 | |
| chr20:62,142,789–62,144,593 | 223.8 kb | Distal (>10kb) Multiome | 841 | |
| chr20:62,182,546–62,183,326 | 184.3 kb | Distal (>10kb) Multiome | 1075 | |
| chr20:62,219,369–62,220,936 | 147.0 kb | Distal (>10kb) Multiome | 599 | |
| chr20:62,237,918–62,239,210 | 128.9 kb | Distal (>10kb) Multiome | 794 | |
| chr20:62,301,857–62,303,576 | 65.1 kb | Distal (>10kb) Multiome | 1110 | |
| chr20:62,361,881–62,362,135 | 5.2 kb | Proximal (<10kb) | 236 | |
| chr20:62,365,805–62,369,404 | 1.7 kb | Proximal (<10kb) Multiome | 960 | |
| chr20:62,376,352–62,376,776 | 9.0 kb | Proximal (<10kb) | 607 | |
| chr20:62,386,553–62,387,309 | 19.7 kb | Distal (>10kb) Multiome | 885 | |
| chr20:62,406,793–62,407,817 | 40.0 kb | Distal (>10kb) Multiome | 935 | |
| chr20:62,475,060–62,476,807 | 108.7 kb | Distal (>10kb) Multiome | 378 | |
| chr20:62,487,549–62,488,006 | 120.4 kb | Distal (>10kb) Multiome | 316 | |
| chr20:62,641,913–62,643,114 | 275.1 kb | Distal (>10kb) Multiome | 874 |
Genomic view of the LAMA5 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.