This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012]
Transcription factors with Perturb-seq knockdown data for KRT8. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = KRT8 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of KRT8, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr12:52,714,144–52,714,803 | 235.4 kb | Distal (>10kb) Multiome | 145 | |
| chr12:52,879,361–52,880,433 | 70.0 kb | Distal (>10kb) Multiome | 702 | |
| chr12:52,884,158–52,885,336 | 65.0 kb | Distal (>10kb) Multiome | 610 | |
| chr12:52,903,331–52,905,680 | 46.2 kb | Distal (>10kb) Multiome HiCAR | 985 | |
| chr12:52,906,476–52,907,649 | 42.6 kb | Distal (>10kb) Multiome HiCAR | 424 | |
| chr12:52,945,414–52,946,146 | 4.1 kb | Proximal (<10kb) Multiome | 514 | |
| chr12:52,947,826–52,948,366 | 1.5 kb | Proximal (<10kb) | 114 | |
| chr12:52,948,516–52,950,156 | 907 bp | At TSS Multiome | 923 | |
| chr12:52,957,069–52,957,367 | 7.2 kb | Proximal (<10kb) | 109 | |
| chr12:53,005,438–53,007,356 | 56.4 kb | Distal (>10kb) Multiome | 995 | |
| chr12:53,046,385–53,049,295 | 97.5 kb | Distal (>10kb) Multiome | 793 | |
| chr12:53,049,465–53,050,530 | 100.1 kb | Distal (>10kb) Multiome | 565 | |
| chr12:53,053,508–53,054,860 | 104.6 kb | Distal (>10kb) Multiome | 646 | |
| chr12:53,078,790–53,079,991 | 129.5 kb | Distal (>10kb) Multiome | 835 | |
| chr12:53,097,544–53,098,160 | 147.9 kb | Distal (>10kb) Multiome | 515 | |
| chr12:53,174,900–53,175,484 | 225.4 kb | Distal (>10kb) Multiome | 474 | |
| chr12:53,179,987–53,181,456 | 230.8 kb | Distal (>10kb) Multiome | 930 | |
| chr12:53,219,787–53,222,151 | 271.2 kb | Distal (>10kb) Multiome | 828 | |
| chr12:53,231,703–53,233,098 | 282.5 kb | Distal (>10kb) Multiome | 464 |
Genomic view of the KRT8 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.