The transport of molecules between the nucleus and the cytoplasm in eukaryotic cells is mediated by the nuclear pore complex (NPC), which consists of 60-100 proteins. Small molecules (up to 70 kD) can pass through the nuclear pore by nonselective diffusion while larger molecules are transported by an active process. The protein encoded by this gene belongs to the importin alpha family, and is involved in nuclear protein import. This protein interacts with the recombination activating gene 1 (RAG1) protein and is a putative substrate of the RAG1 ubiquitin ligase. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2012]
Transcription factors with Perturb-seq knockdown data for KPNA1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = KPNA1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of KPNA1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr3:122,382,880–122,383,692 | 131.8 kb | Distal (>10kb) Multiome | 711 | |
| chr3:122,383,843–122,384,747 | 130.7 kb | Distal (>10kb) Multiome | 937 | |
| chr3:122,442,936–122,443,707 | 71.6 kb | Distal (>10kb) Multiome | 134 | |
| chr3:122,505,755–122,506,052 | 8.9 kb | Proximal (<10kb) | 31 | |
| chr3:122,514,019–122,515,266 | 92 bp | At TSS Multiome | 888 | |
| chr3:122,564,086–122,564,854 | 49.5 kb | Distal (>10kb) Multiome | 849 | |
| chr3:122,680,509–122,681,348 | 166.1 kb | Distal (>10kb) Multiome HiCAR | 567 | |
| chr3:122,798,643–122,799,856 | 284.3 kb | Distal (>10kb) Multiome | 131 |
Genomic view of the KPNA1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.