Transcription factors with Perturb-seq knockdown data for KLRK1-AS1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = KLRK1-AS1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of KLRK1-AS1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr12:10,153,098–10,154,282 | 214.6 kb | Distal (>10kb) Multiome | 400 | |
| chr12:10,211,987–10,214,332 | 155.7 kb | Distal (>10kb) Multiome | 873 | |
| chr12:10,361,067–10,361,431 | 6.9 kb | Proximal (<10kb) | 87 | |
| chr12:10,363,919–10,365,484 | 2.8 kb | Proximal (<10kb) | 514 | |
| chr12:10,557,141–10,558,180 | 189.3 kb | Distal (>10kb) Multiome | 180 | |
| chr12:10,613,109–10,614,787 | 245.3 kb | Distal (>10kb) Multiome | 875 |
Genomic view of the KLRK1-AS1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.