This gene encodes a protein that belongs to the kelch repeat-containing family, and contains an N-terminal BTB/POZ domain, a BACK domain and six C-terminal kelch repeats. The encoded protein is a component of a complex with cullin 3-based E3 ligase, which plays a role in mitosis. This protein complex is a cell cycle regulator, and functions in the organization and integrity of the spindle midzone in anaphase and the completion of cytokinesis. The complex is required for the removal of the chromosomal passenger protein aurora B from mitotic chromosomes. [provided by RefSeq, Jul 2016]
Transcription factors with Perturb-seq knockdown data for KLHL9. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = KLHL9 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of KLHL9, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr9:21,326,676–21,326,840 | 8.6 kb | Proximal (<10kb) | 72 | |
| chr9:21,334,615–21,335,758 | 9 bp | At TSS Multiome | 770 | |
| chr9:21,402,228–21,403,414 | 67.5 kb | Distal (>10kb) Multiome | 255 | |
| chr9:21,504,615–21,505,684 | 169.7 kb | Distal (>10kb) Multiome | 144 | |
| chr9:21,559,092–21,560,760 | 224.4 kb | Distal (>10kb) Multiome | 437 | |
| chr9:21,801,496–21,803,636 | 467.2 kb | Distal (>10kb) Multiome HiCAR | 911 |
Genomic view of the KLHL9 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.